Review





Similar Products

kdr  (Miltenyi Biotec)
94
Miltenyi Biotec kdr
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Kdr, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kdr/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
kdr - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
anti vegfr  (Sino Biological)
94
Sino Biological anti vegfr
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Anti Vegfr, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vegfr/product/Sino Biological
Average 94 stars, based on 1 article reviews
anti vegfr - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
vegfr2  (Boster Bio)
94
Boster Bio vegfr2
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Vegfr2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vegfr2/product/Boster Bio
Average 94 stars, based on 1 article reviews
vegfr2 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
boster biological technology co  (Boster Bio)
93
Boster Bio boster biological technology co
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Boster Biological Technology Co, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/boster biological technology co/product/Boster Bio
Average 93 stars, based on 1 article reviews
boster biological technology co - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
anti vegfr2  (Boster Bio)
94
Boster Bio anti vegfr2
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Anti Vegfr2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vegfr2/product/Boster Bio
Average 94 stars, based on 1 article reviews
anti vegfr2 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
kdr vegfr2  (Proteintech)
96
Proteintech kdr vegfr2
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Kdr Vegfr2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kdr vegfr2/product/Proteintech
Average 96 stars, based on 1 article reviews
kdr vegfr2 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
igg1 fitc kdr flk1 miltenyi biotec  (Miltenyi Biotec)
94
Miltenyi Biotec igg1 fitc kdr flk1 miltenyi biotec
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Igg1 Fitc Kdr Flk1 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igg1 fitc kdr flk1 miltenyi biotec/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
igg1 fitc kdr flk1 miltenyi biotec - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
vegfr2 kdr  (Proteintech)
96
Proteintech vegfr2 kdr
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Vegfr2 Kdr, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vegfr2 kdr/product/Proteintech
Average 96 stars, based on 1 article reviews
vegfr2 kdr - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
kdr  (Proteintech)
96
Proteintech kdr
Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , <t>KDR</t> , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial <t>(</t> <t>PECAM1</t> ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.
Kdr, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kdr/product/Proteintech
Average 96 stars, based on 1 article reviews
kdr - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , KDR , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial ( PECAM1 ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Tissue-resident macrophages co-develop with myocardial tissue in human induced pluripotent stem cell-derived organoids

doi: 10.3389/fcell.2025.1629988

Figure Lengend Snippet: Cardiac Organoids. (A) Schematic representation of the cardiac organoid differentiation protocol displaying experimental timing, cell culture media as well as small molecules and cytokines used to induce cardiac tissue. The image was created in BioRender (Wörsdörfer, P. (2025) https://BioRender.com/rz8x29g ). (B) Semiquantitative RT-PCR detecting mesodermal ( TBXT , KDR , GATA4 , RUNX1 ), cardiac ( NKX2.5 , TBX5 , ISL1 , TNNT2 , DES , RYR2 ), and endothelial ( PECAM1 ) marker gene expression at days 2, 4 and 8 of differentiation. (C) H&E staining of an organoid section at day 2. (D,D′) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers TBXT and VIM at day 2 of organoid development. (E) Immunofluorescence analysis of the organoid shown in C detecting early mesodermal markers GATA6 and KDR at day 2 of organoid development. (F) H&E staining showing hollow cavity-like structures in cardiac organoids at day 7. (G,G′) Immunofluorescence analysis of the organoid shown in F detecting mesenchymal cells (VIM + ) in close association with cardiomyocytes (TNNT2 + ) at day 7 of organoid development. (H) H&E staining of organoid sections at day 35 of development. (I) Immunofluorescence analysis of the organoid shown in H showing close interaction of fibroblasts (VIM + ) and cardiomyocytes (TNNT2 + ) at day 35 of organoid development. (J) Cardiac organoids progressively increase in size (day 0 = 712.2 ± 6.8 µm, n = 72; day 7 = 1,231 ± 20.3 µm, n = 70; day 25 = 1,613 ± 45.4 µm, n = 41. ***p < 0.001, ****p < 0.0001. (K) Contraction frequency of organoids increases over time, from 25 ± 1.9 bpm at day 7–57 ± 10.5 bpm at day 35 (n = 8). **p < 0.01, ****p < 0.0001.

Article Snippet: Primary antibodies against PECAM1 (CD31) (DAKO, M0823), VIM (Invitrogen, MA5-16409), ACTN1 (Abcam, Ab68167), KDR (Miltenyi Biotec, 130-125-988), TBXT (R&D Systems, AF2085), TNNT2 (Life Technologies, MA512960 ), CD34 (Life Technologies, MA5-32059), AIF1 (WAKO/Fuji Film, 019-19741) and PTPRC (CD45) (Life Technologies, 14-0451-82) were diluted in NBS blocking solution and incubated overnight at 4 °C.

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Marker, Gene Expression, Staining, Immunofluorescence